Relationship between Human Papillomavirus Prevalence and DNA Damage in Cervical Cancer Population in Gansu Province, China

Zhong Guo; Tianyuan Zhu; Yunsong Zhou; Tianbin Si; Qiang Wang; Hongmei Qu; Chenjing Wang; Jin Zhao

doi:10.1159/000525975

Relationship between Human Papillomavirus Prevalence and DNA Damage in Cervical Cancer Population in Gansu Province, China

Intervirology. 2022;65(4):215-223. doi: 10.1159/000525975. Epub 2022 Jul 12.

Authors

Zhong Guo^{1

2}, Tianyuan Zhu³, Yunsong Zhou^{1

2}, Tianbin Si⁴, Qiang Wang⁵, Hongmei Qu^{1

2}, Chenjing Wang^{1

2}, Jin Zhao^{1

2}

Affiliations

¹ Medical College of Northwest Minzu University, Lanzhou, China.
² Key Laboratory of Environmental Ecology and Population Health in Northwest Minority Areas, Northwest Minzu University, Lanzhou, China.
³ Gansu Provincial Maternity and Child-care Hospital, Lanzhou, China.
⁴ Gansu Provincial Cancer Hospital, Lanzhou, China.
⁵ No. 1 Hospital of Longnan City, Longnan, China.

Abstract

Objectives: The aim of this study was to assess the possible reason of the high incidence and mortality of cervical cancer in Longnan, China.

Materials and methods: 147 and 124 invasive squamous-cell carcinoma (SCC) samples from Longnan and different cities and districts of Gansu province were collected in the present study. All the samples were obtained from patients who underwent biopsies with colposcopy or advanced operations and were evaluated by experienced pathologists. HPV genotypes were examined with a validated HPV subtypes kit. The prevalence of HPV infection in SCC patients of China was analyzed by evidence-based medicine in the published literature. The markers of DNA damage response (DDR) - ATMpSer1981, H2AXp Ser139 (γH2AX), Chk2pThr68, and p53 - were analyzed by immunohistochemistry.

Results: HPV positivity, high-risk and multiple HPV positivity, and HPV58 infection were significantly higher in Longnan. Our results show that the prevalence of HPV infection in SCC patients of Longnan are consistent with the HPV prevalence in China. ATM, γH2AX, and p53 expressions in total and HPV+ samples were also higher in Longnan.

Conclusions: HPV-related DDR activation may be one reason for the high incidence and mortality of Longnan cervical cancer.

Keywords: Cervical cancer; DNA damage response; Human papillomaviruses.

MeSH terms

Alphapapillomavirus*
Carcinoma*
China / epidemiology
DNA Damage
Female
Genotype
Humans
Papillomaviridae / genetics
Papillomavirus Infections* / epidemiology
Prevalence
Tumor Suppressor Protein p53 / genetics
Uterine Cervical Neoplasms*

Substances

Tumor Suppressor Protein p53

Grants and funding

This study was supported by the Fundamental Research Funds for the Central Universities (No. 31920170164 and 31920190210), the Gansu Province Natural Science Foundation (20YF8FA046), and the National Natural Science Foundation of China (81260442, 82160509).