Calculation of Fetal Fraction for Non-Invasive Prenatal Testing

BioTech (Basel). 2021 Aug 9;10(3):17. doi: 10.3390/biotech10030017.


Estimating the fetal fraction of DNA in a pregnant mother's blood is a risk-free, non-invasive way of predicting fetal aneuploidy. It is a rapidly developing field of study, offering researchers a plethora of different complementary methods. Such methods include examining the differences in methylation profiles between the fetus and the mother. Others include calculating the average allele frequency based on the difference in genotype of a number of single-nucleotide polymorphisms. Differences in the length distribution of DNA fragments between the mother and the fetus as well as measuring the proportion of DNA reads mapping to the Y chromosome also constitute fetal fraction estimation methods. The advantages and disadvantages of each of these main method types are discussed. Moreover, several well-known fetal fraction estimation methods, such as SeqFF, are described and compared with other methods. These methods are amenable to not only the estimation of fetal fraction but also paternity, cancer, and transplantation monitoring studies. NIPT is safe, and should aneuploidy be detected, this information can help parents prepare mentally and emotionally for the birth of a special needs child.

Keywords: NIPT; SNP quantification; SeqFF; Y chromosome; aneuploidy; cfDNA; differential methylation; fetal fraction; length distribution estimation.

Publication types

  • Review