PhePLATZ1, a PLATZ transcription factor in moso bamboo (Phyllostachys edulis), improves drought resistance of transgenic Arabidopsis thaliana

Plant Physiol Biochem. 2022 Sep 1:186:121-134. doi: 10.1016/j.plaphy.2022.07.004. Epub 2022 Jul 8.

Abstract

Drought is one of the most serious environmental stresses. Plant AT-rich sequence and zinc-binding (PLATZ) proteins perform indispensable functions to regulate plant growth and development and to respond to environmental stress. In this present study, we identified PhePLATZ1 in moso bamboo and found that its expression was up-regulated in response to 20% PEG-6000 and abscisic acid (ABA) treatments. Next, transgenic PhePLATZ1-overexpressing Arabidopsis lines were generated. Overexpression of PhePLATZ1 improved drought stress resistance of transgenic plants by mediating osmotic regulation, enhancing water retention capacity and reducing membrane and oxidative damage. These findings were corroborated by analysing physiological indicators including chlorophyll, relative water content, leaf water loss rate, electrolyte leakage, H2O2, proline, malondialdehyde content and the enzyme activities of peroxidase and catalase. Subsequent seed germination and seedling root length experiments that included exposure to exogenous ABA treatments showed that ABA sensitivity decreased in transgenic plants relative to wild-type plants. Moreover, transgenic PhePLATZ1-overexpressing plants promoted stomatal closure in response to ABA treatment, suggesting that PhePLATZ1 might play a positive regulatory role in the drought resistance of plants via the ABA signaling pathway. In addition, the transgenic PhePLATZ1-OE plants showed altered expression of some stress-related genes when grown under drought conditions. Taken together, these findings improve our understanding of the drought response of moso bamboo and provide a key candidate gene for the molecular breeding of this species for drought tolerance.

Keywords: ABA signaling; Drought tolerance; Moso bamboo; PhePLATZ1; Stomatal closure.

MeSH terms

  • Abscisic Acid / metabolism
  • Abscisic Acid / pharmacology
  • Arabidopsis* / metabolism
  • Droughts*
  • Gene Expression Regulation, Plant
  • Hydrogen Peroxide / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified / metabolism
  • Poaceae / genetics
  • Poaceae / metabolism
  • Stress, Physiological / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Water / metabolism

Substances

  • Plant Proteins
  • Transcription Factors
  • Water
  • Abscisic Acid
  • Hydrogen Peroxide