Preparation of 111In leukocytes after hemolytic removal of erythrocytes

Int J Rad Appl Instrum B. 1987;14(1):79-80. doi: 10.1016/0883-2897(87)90166-8.


An optimized procedure is described for isolation and high-efficiency radiolabeling of leukocytes using 111In-oxine. The chief advantages over conventional methods include virtually no loss of leukocytes during washing and separation steps; a significant reduction in the time required to prepare leukocytes for radiolabeling compared to non-hemolytic preparations; a 28% increase in the average labeling efficiency obtained using 111In-oxine; greater than 95% cell viability as measured by the trypan blue exclusion test; elimination of contaminating red blood cells from the leukocyte pellet prior to labeling; and 80% survivability at 15 min post injection (measured as per cent of blood activity on leukocyte fraction).

MeSH terms

  • Cell Separation
  • Erythrocytes
  • Hemolysis
  • Humans
  • Indium*
  • Isotope Labeling
  • Leukocytes*
  • Radioisotopes*


  • Radioisotopes
  • Indium