Frequent detection of monkeypox virus DNA in saliva, semen, and other clinical samples from 12 patients, Barcelona, Spain, May to June 2022

Euro Surveill. 2022 Jul;27(28):2200503. doi: 10.2807/1560-7917.ES.2022.27.28.2200503.


A monkeypox (MPX) outbreak has expanded worldwide since May 2022. We tested 147 clinical samples collected at different time points from 12 patients by real-time PCR. MPX DNA was detected in saliva from all cases, sometimes with high viral loads. Other samples were frequently positive: rectal swab (11/12 cases), nasopharyngeal swab (10/12 cases), semen (7/9 cases), urine (9/12 cases) and faeces (8/12 cases). These results improve knowledge on virus shedding and the possible role of bodily fluids in disease transmission.

Keywords: PCR; monkeypox; outbreak; saliva; semen; shedding.

MeSH terms

  • DNA, Viral / genetics
  • Humans
  • Monkeypox virus* / isolation & purification
  • Monkeypox* / diagnosis
  • Monkeypox* / epidemiology
  • Saliva
  • Semen
  • Spain / epidemiology


  • DNA, Viral