Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases

Paul C Guest; Mitra Abbasifard; Tannaz Jamialahmadi; Muhammed Majeed; Prashant Kesharwani; Amirhossein Sahebkar

doi:10.1007/978-1-0716-2395-4_18

Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases

Methods Mol Biol. 2022:2511:245-256. doi: 10.1007/978-1-0716-2395-4_18.

Authors

Paul C Guest¹, Mitra Abbasifard^{2

3}, Tannaz Jamialahmadi⁴, Muhammed Majeed⁵, Prashant Kesharwani⁶, Amirhossein Sahebkar^{7

8

9

10}

Affiliations

¹ Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
² Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. abbasifardm@yandex.com.
³ Department of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. abbasifardm@yandex.com.
⁴ Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
⁵ Sabinsa Corporation, East Windsor, NJ, USA.
⁶ Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
⁷ Applied Biomeical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. sahebkara@mums.ac.ir.
⁸ Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. sahebkara@mums.ac.ir.
⁹ School of Medicine, The University of Western Australia, Perth, Australia. sahebkara@mums.ac.ir.
¹⁰ School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. sahebkara@mums.ac.ir.

PMID: 35838965
DOI: 10.1007/978-1-0716-2395-4_18

Abstract

Severe cases of SARS-CoV-2 and other pathogenic virus infections are often associated with the uncontrolled release of proinflammatory cytokines, known as a "cytokine storm." We present a protocol for multiplex analysis of three cytokines, tumor necrosis factor-alpha (TNF-a), interleukin 6 (IL-6), and IL-10, which are typically elevated in cytokine storm events and may be used as a predictive biomarker profile of disease severity or disease course.

Keywords: COVID-19; Cytokine storm; IL-10; IL-6; Multiplex immunoassay; SARS-CoV-2; TNF-α.

MeSH terms

COVID-19* / diagnosis
Cytokine Release Syndrome* / diagnosis
Cytokines
Humans
Immunoassay / methods
SARS-CoV-2
Severity of Illness Index

Substances

Cytokines