Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment

Krist Helen Antunes; Ana Paula Duarte de Souza

doi:10.1007/978-1-0716-2395-4_28

Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment

Methods Mol Biol. 2022:2511:367-373. doi: 10.1007/978-1-0716-2395-4_28.

Authors

Krist Helen Antunes¹, Ana Paula Duarte de Souza²

Affiliations

¹ Laboratory of Clinical and Experimental Immunology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
² Laboratory of Clinical and Experimental Immunology, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. ana.duarte@pucrs.br.

PMID: 35838975
DOI: 10.1007/978-1-0716-2395-4_28

Abstract

The use of in vitro methods of infecting cell lines to test new treatments for SARS-CoV-2 does not always recapitulate the real context of the infection, and mouse models for SARS-CoV-2 infection are limited. Here we describe a novel ex vivo approach by collecting, isolating, and culturing nasal epithelial cells obtained from patients with COVID-19. This technique allows us to study immune responses and test new treatments directly on cells from patients naturally infected with SARS-CoV-2.

Keywords: COVID-19; Cell culture; Ex vivo; Isolation; Nasopharyngeal cells; SARS-CoV-2.

MeSH terms

Animals
Antiviral Agents
COVID-19*
Cell Culture Techniques
Humans
Immunity
Mice
SARS-CoV-2*

Substances

Antiviral Agents