Investigation of physicochemical properties, antimicrobial and antioxidant activity of edible films based on chitosan/casein containing Origanum vulgare L. essential oil and its effect on quality maintenance of cherry tomato

Food Chem. 2022 Dec 1:396:133650. doi: 10.1016/j.foodchem.2022.133650. Epub 2022 Jul 8.


Edible coatings prevent physicochemical and biological food deterioration. Using bioactive compounds like essential oils can enrich films. In this study, edible films from chitosan (C), casein (Z) and oregano (OEO) were developed, and their physicochemical, barrier, antimicrobial, antioxidant, and structural properties (FTIR, SEM) were investigated. The C1Z3 ratio had good mechanical and inhibitory properties, and OEO improves flexibility, barrier, hydrophobic, antimicrobial, and antioxidant properties. The physicochemical and microbiological properties of cherry tomatoes were affected by C1Z3 and C1Z3O1.5 coatings. Coated fruits were stored at 4 °C for 32 days. The best results for weight loss, shrinkage, and titratable acidity were found to be 17.88%, 31.12%, and 0.15% in C1Z3O1.5 coated cherry tomatoes, respectively.The TMAB of C1Z3O1.5 coated fruits was less than detectable and the fungal growth was inhibited for 28 days. Accordingly, by adding OEO to chitosan/casein coatings, the spoilage process of cherry tomatoes was delayed for long-term storage.

Keywords: Antifungal activity; Casein; Cherry tomato; Chitosan; Edible film; Origanum vulgare L.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Infective Agents* / chemistry
  • Anti-Infective Agents* / pharmacology
  • Antioxidants / pharmacology
  • Caseins
  • Chitosan* / chemistry
  • Edible Films*
  • Oils, Volatile* / chemistry
  • Oils, Volatile* / pharmacology
  • Origanum* / chemistry
  • Solanum lycopersicum* / microbiology


  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Antioxidants
  • Caseins
  • Oils, Volatile
  • Chitosan