The Mechanism of Rac1 in Regulating HCC Cell Glycolysis Which Provides Underlying Therapeutic Target for HCC Therapy

Yin-Xiang Ren; Xiao-Bin Li; Wei Liu; Xu-Guang Yang; Xin Liu; Yu Luo

doi:10.1155/2022/7319641

The Mechanism of Rac1 in Regulating HCC Cell Glycolysis Which Provides Underlying Therapeutic Target for HCC Therapy

J Oncol. 2022 Jul 6:2022:7319641. doi: 10.1155/2022/7319641. eCollection 2022.

Authors

Yin-Xiang Ren¹, Xiao-Bin Li¹, Wei Liu¹, Xu-Guang Yang¹, Xin Liu², Yu Luo¹

Affiliations

¹ School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
² School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

Abstract

Aim: To explore the role of Rac1 on sorafenib resistance in hepatocellular carcinoma.

Methods: CCK-8, wound healing assay, Transwell, and cell cycle assay were used to detect the tumor cells development. Cell viability was assessed by MTT. The glycolytic pathway was revealed by cellular metabolism assays.

Result: We recovered that Rac1 upregulation was related to HCC patients' poorer prognosis. Forced expression of Rac1 promoted cell development and sorafenib chemoresistance in HCC cells. Rac1 inhibitor EHop-016 and sorafenib combination markedly prevented cell viability, G2/M phase cycle arrest, and apoptosis than single therapy. Furthermore, combination therapy decreased glycolysis in HCC cells. In vivo, the tumor growth was significantly prevented by combination therapy single therapy.

Conclusion: Our research declares that Rac1 inhibition could block sorafenib resistance in HCC by decreasing glycolysis, which would provide an underlying target for HCC therapy.