Efficacy of multi-strain probiotic along with dietary and lifestyle modifications on polycystic ovary syndrome: a randomised, double-blind placebo-controlled study

Eur J Nutr. 2022 Dec;61(8):4145-4154. doi: 10.1007/s00394-022-02959-z. Epub 2022 Jul 20.


Purpose: Effect of multi-strain probiotic along with dietary and lifestyle modifications in the management of polycystic ovary syndrome (PCOS) has rarely been reported. We thus aimed to investigate the effect of multi-strain probiotic (Lactobacillus acidophilus UBLA-34, L. rhamnosus UBLR-58, L. reuteri UBLRu-87 (each of 2 billion colony forming units (CFU)); L. plantarum UBLP-40, L. casei UBLC-42, L. fermentum UBLF-31, Bifidobacterium bifidum UBBB-55 (each of 1 billion CFU) and fructo-oligosaccharides (100 mg)) and dietary and lifestyle modifications on restoration of menstrual regularity, weight reduction, metabolic and hormonal profile in women with PCOS.

Methods: A 104 participants (age 18-40 years) were randomly allocated to receive probiotic or placebo capsules for 6 months. Baseline and end line assessment were performed for menstrual cycle regularity, ultrasonography scan for ovaries, total testosterone, dehydroepiandrosterone (DHEAS), insulin, luteinizing hormone/follicle stimulating hormone (LH/FSH) ratio, fasting blood sugar (FBS), homeostatic model assessment-insulin resistance (HOMA-IR), weight reduction, waist-/hip circumference (WC, HC), waist to hip ratio (WHR), and body mass index (BMI). Plasma lipopolysaccharide and effect of intervention on quality of life was investigated. Diet and exercise were controlled during the trial.

Results: Probiotic supplement along with dietary and lifestyle modifications significantly regularised menstrual cycle (p 0.023), improved levels of total testosterone (p 0.043), WC (p 0.030), WHR (p 0.027) and menstrual domain of quality of life (p 0.034) as compared to placebo. No adverse events related to study were reported.

Conclusion: Multi-strain probiotic along with dietary and lifestyle modifications were effective in the management of PCOS.

Trial registration: CTRI: CTRI/2016/07/007086, dated 13 July 2016.

Keywords: Lipopolysaccharide; Menstrual cycle; PCOS; Probiotic; Testosterone.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adolescent
  • Adult
  • Blood Glucose / metabolism
  • Body Mass Index
  • Dehydroepiandrosterone
  • Diet*
  • Female
  • Follicle Stimulating Hormone
  • Humans
  • Insulin
  • Insulin Resistance
  • Life Style*
  • Lipopolysaccharides
  • Luteinizing Hormone
  • Polycystic Ovary Syndrome* / therapy
  • Probiotics* / therapeutic use
  • Quality of Life
  • Testosterone
  • Weight Loss
  • Young Adult


  • Blood Glucose
  • Dehydroepiandrosterone
  • Follicle Stimulating Hormone
  • Insulin
  • Lipopolysaccharides
  • Luteinizing Hormone
  • Testosterone

Associated data

  • CTRI/CTRI/2016/07/007086