Enhancing GAT-3 in thalamic astrocytes promotes resilience to brain injury in rodents

Sci Transl Med. 2022 Jul 6;14(652):eabj4310. doi: 10.1126/scitranslmed.abj4310. Epub 2022 Jul 6.


Inflammatory processes induced by brain injury are important for recovery; however, when uncontrolled, inflammation can be deleterious, likely explaining why most anti-inflammatory treatments have failed to improve neurological outcomes after brain injury in clinical trials. In the thalamus, chronic activation of glial cells, a proxy of inflammation, has been suggested as an indicator of increased seizure risk and cognitive deficits that develop after cortical injury. Furthermore, lesions in the thalamus, more than other brain regions, have been reported in patients with viral infections associated with neurological deficits, such as SARS-CoV-2. However, the extent to which thalamic inflammation is a driver or by-product of neurological deficits remains unknown. Here, we found that thalamic inflammation in mice was sufficient to phenocopy the cellular and circuit hyperexcitability, enhanced seizure risk, and disruptions in cortical rhythms that develop after cortical injury. In our model, down-regulation of the GABA transporter GAT-3 in thalamic astrocytes mediated this neurological dysfunction. In addition, GAT-3 was decreased in regions of thalamic reactive astrocytes in mouse models of cortical injury. Enhancing GAT-3 in thalamic astrocytes prevented seizure risk, restored cortical states, and was protective against severe chemoconvulsant-induced seizures and mortality in a mouse model of traumatic brain injury, emphasizing the potential of therapeutically targeting this pathway. Together, our results identified a potential therapeutic target for reducing negative outcomes after brain injury.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Astrocytes / metabolism
  • Brain Injuries*
  • COVID-19*
  • Disease Models, Animal
  • GABA Plasma Membrane Transport Proteins / metabolism
  • Inflammation / pathology
  • Mice
  • Polymers
  • Rodentia / metabolism
  • SARS-CoV-2
  • Seizures
  • Thalamus / metabolism
  • Thalamus / pathology


  • GABA Plasma Membrane Transport Proteins
  • Polymers
  • GAT