An Improved Surgical Technique to Increase Neck Width of Elastase-Induced Aneurysm Model in Rabbits: A Prospective Study

Yang Zhang; Yu He; ChaoJie Tang; YuFan Wu; Yi Gu; BinXian Gu; Li Chen; WenWei Gao; ZhiGuo Zhou; YouKe Qi; FaJiang Mao; YongNing Sun; Wu Wang

doi:10.3389/fneur.2022.889140

An Improved Surgical Technique to Increase Neck Width of Elastase-Induced Aneurysm Model in Rabbits: A Prospective Study

Front Neurol. 2022 Jul 1:13:889140. doi: 10.3389/fneur.2022.889140. eCollection 2022.

Authors

Yang Zhang¹, Yu He¹, ChaoJie Tang¹, YuFan Wu¹, Yi Gu¹, BinXian Gu¹, Li Chen¹, WenWei Gao², ZhiGuo Zhou³, YouKe Qi⁴, FaJiang Mao⁴, YongNing Sun⁴, Wu Wang¹

Affiliations

¹ Institute of Diagnostic and Interventional Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
² Neurosurgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
³ Joint International Research Laboratory of Resource Chemistry, Ministry of Education, Shanghai Normal University, Shanghai, China.
⁴ Department of Cardiology, Shanghai Municipal Hospital of Tradition Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Abstract

Background: Rabbit elastase-induced aneurysms have widely been used to test various endovascular materials over the past two decades. However, wide-necked aneurysms cannot be stably constructed.

Objective: The purpose of the study was to increase the neck width of the elastase-induced aneurysm model in rabbits via an improved surgical technique with two temporary clips.

Materials and methods: Fifty-four elastase-induced aneurysms in rabbits were successfully created. Group 1 was (n = 34) composed of cases in which two temporary aneurysm clips were placed closely medially and laterally to the origin of the right common carotid artery (RCCA), respectively. Group 2 (n = 20) included cases in which a single temporary aneurysm clip was placed crossed the origin of RCCA. Digital subtraction angiography (DSA) was performed before and immediately after elastase incubation and 3 weeks later. The diameter of the origin of RCCA before and immediately after elastase incubation and aneurysm sizes of the two groups were measured and compared. Moreover, the correlation analysis was performed between the diameter of the origin of RCCA immediately after elastase incubation and aneurysm neck width.

Results: The mean aneurysm neck and dome width of group 1 were both significantly larger than that of group 2 (p-value < 0.001 and p-value = 0.005, respectively). Moreover, the proportion of wide-necked aneurysms (neck width ≥4 mm) in group 1 was significantly larger than that in group 2 (p-value = 0.004) and the mean dome to neck ratio (D/N) of group 1 was smaller than that of group 2 (p-value = 0.008). Furthermore, there was a positive correlation between the diameter of the origin of RCCA immediately after elastase incubation and aneurysm neck width.

Conclusion: The improved surgical technique with two temporary clips, focusing on the direct contact of elastase with the origin of RCCA, could increase the neck width of elastase-induced aneurysm models in rabbits.

Keywords: aneurysm model; aneurysm neck width; elastase; rabbit; temporary aneurysm clips.