Time-course of the human thoracic aorta ageing process assessed using uniaxial mechanical testing and constitutive modelling

Alessandro Giudici; Ye Li; Yasmin; Sarah Cleary; Kathleen Connolly; Carmel McEniery; Ian B Wilkinson; Ashraf W Khir

doi:10.1016/j.jmbbm.2022.105339

Time-course of the human thoracic aorta ageing process assessed using uniaxial mechanical testing and constitutive modelling

J Mech Behav Biomed Mater. 2022 Oct:134:105339. doi: 10.1016/j.jmbbm.2022.105339. Epub 2022 Jul 4.

Authors

Alessandro Giudici¹, Ye Li², Yasmin³, Sarah Cleary³, Kathleen Connolly³, Carmel McEniery³, Ian B Wilkinson³, Ashraf W Khir⁴

Affiliations

¹ Brunel Institute for Bioengineering, Brunel University London, Kingston Lane, Uxbridge, UB8 3PH, UK; Department of Biomedical Engineering, CARIM School for Cardiovascular Diseases, Maastricht University, 6229 ER, the Netherlands.
² Brunel Institute for Bioengineering, Brunel University London, Kingston Lane, Uxbridge, UB8 3PH, UK; British Heart Foundation Centre, King's College London, Strand, London, WC2R 2LS, UK.
³ Division of Experimental Medicine and Immunotherapeutics, University of Cambridge, Hills Road, Cambridge, CB2 0QO, UK.
⁴ Brunel Institute for Bioengineering, Brunel University London, Kingston Lane, Uxbridge, UB8 3PH, UK; Department of Engineering, Durham University, Durham, DH1 3LE, UK. Electronic address: ashraf.w.khir@durham.ac.uk.

PMID: 35868063
DOI: 10.1016/j.jmbbm.2022.105339

Abstract

Age-related remodelling of the arterial wall shifts the load bearing from the compliant elastin network to the stiffer collagen fibres. While this phenomenon has been widely investigated in animal models, human studies are lacking due to shortage of donors' arteries. This work aimed to characterise the effect of ageing on the mechanical properties of the human aortic wall in the circumferential direction. N = 127 thoracic aortic rings (age 18-81 years) were subjected to circumferential tensile testing. The tangential elastic modulus (K_θθθθ) was calculated at pressure-equivalent stresses ranging 60-100 mmHg. Further, the mechanical data were fitted using the Holzpafel-Gasser-Ogden hyperelastic strain energy function (HGO-SEF), modelling the superimposed response of an isotropic matrix (elastin) reinforced by collagen fibres. K_θθθθ increased with age across at all considered pressures (p < 0.001), although more strongly at higher pressures. Indeed, the slope of the linear K_θθθθ-pressure relationship increased by 300% from donors <30 to ≥70 years (4.72± 2.95 to 19.06± 6.82 kPa/mmHg, p < 0.001). The HGO-SEF elastin stiffness-like parameter dropped by 31% between 30 and 40 years (p < 0.05) with non-significant changes thereafter. Conversely, changes in HGO-SEF collagen parameters were observed later at age>60 years, with the exponential constant increasing by ∼20-50 times in the investigated age range (p < 0.001). The results provided evidence that the human thoracic aorta undergoes stiffening during its life-course. Constitutive modelling suggested that these changes in arterial mechanics are related to the different degeneration time-courses of elastin and collagen; likely due to considerable fragmentation of elastin first, with the load bearing shifting from the compliant elastin to the stiffer collagen fibres. This process leads to a gradual impairment of the aortic elastic function with age.

Keywords: Aorta; Arterial ageing; Arterial stiffness; Collagen; Constitutive modelling; Elastin.

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Aging
Animals
Aorta, Thoracic* / physiology
Biomechanical Phenomena
Collagen
Elastin* / physiology
Humans
Mechanical Tests
Middle Aged
Stress, Mechanical
Young Adult

Substances

Collagen
Elastin