As the second most common gynecological tumor in the world, cervical cancer has always been a female killer.Some studies have shown that miR-218 can inhibit the invasion and migration of cervical cancer cells through two target genes, SFMBT1and DCUNIDI. On this basis, this paper presents the study that miR-218 affects the invasion and migration of cervical cancer cells by inhibiting the expression of target genes, SFMBT1 and DCUNIDI. In this paper, we used the control experiment to study. In the fluorescence experiment, we confirmed that SFMBT1 and DCUNIDI are indeed the downstream target genes of miR-218. After that, we down-regulated or up-regulated the expression of SFMBT1 and DCUNIDI in cervical cancer cells through specific small interfering RNA and over-expression plasmids, so we carried out cell migration and cell invasion experiments. The results of the cell invasion experiment showed that the average value of cell invasion ability of the miR-218 group was 286 and 218, that of SFMBT1-siRNA1 was 264, that of DCUNIDI-siRNA1 was 179, that of SFMBT1-siRNA 2 was 245, that of DCUNIDI-siRNA2 was 196, and that of SFMBT1 + miR-218 was 401.The average cell invasiveness of DCUNIDI + miR-218 was 672.