The effects of Aroclor 1254 alone and in combination with X-rays on the male mice germ cells quantity and quality

Abstract

The effects of chemical and physical environmental factors are concerned as the main reason of diminished male fertility. The aim of the study was the investigation of the effects of low doses of Aroclor 1254 or combined exposure to low doses of Aroclor 1254 and low doses of ionizing radiation on the sperm quantity and quality of male germ cells including damage to genetic material of adult male mice. Mice were exposed for 2 weeks, 3 times per week by intraperitoneal injection with Aroclor 1254 diluted in corn oil at doses of 1, 2 and 4 mg/kg bw or to whole body X-rays irradiation at doses 0.05 Gy, 0.10 Gy and 0.15 Gy or to combination of X-rays and Aroclor 1254 at following doses 0.05 Gy + 1 mg/kg bw Aroclor 1254, 0.10 Gy + 2 mg/kg bw Aroclor 1254. The samples for sperm count, motility, morphology and DNA integrity of male germ cells estimation were taken from animals just after the end of exposure and 5 weeks later. Irradiation alone deteriorated sperm count and quality. Aroclor 1254 significantly reduced the sperm motility and increased sperm abnormality and at the highest dose also induced DNA damage of gametes. The combined exposure to 0.10 Gy + 2 mg/kg bw of Aroclor 1254 showed the increase in the sperm concentration and the decrease of percentage of abnormal spermatozoa compared to results after irradiation to 0.10 Gy alone. In conclusion, the low doses of Aroclor 1254 used in this study did not significantly reduce the sperm count, but affected the sperm motility, morphology and sometimes also DNA integrity of gametes. In combination with low doses of irradiation, low doses of Aroclor 1254 may ameliorate the harmful effect of irradiation on the male gametes.

Keywords: Irradiation; Polychlorinated biphenyls; Sperm count and quality.