Long Noncoding XLOC_006390 Regulates the Proliferation and Metastasis of Human Colorectal Cancer via miR-296/ONECUT2 Axis

Tianyi Ma; Tianyu Qiao; Rui Huang; Meng Wang; Hanqing Hu; Ziming Yuan; Yihao Zhu; Hongyu Wu; Xiaoming Zou

doi:10.1155/2022/4897201

Long Noncoding XLOC_006390 Regulates the Proliferation and Metastasis of Human Colorectal Cancer via miR-296/ONECUT2 Axis

J Oncol. 2022 Jul 15:2022:4897201. doi: 10.1155/2022/4897201. eCollection 2022.

Authors

Tianyi Ma¹, Tianyu Qiao¹, Rui Huang¹, Meng Wang¹, Hanqing Hu¹, Ziming Yuan¹, Yihao Zhu¹, Hongyu Wu¹, Xiaoming Zou²

Affiliations

¹ Department of Colorectal Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
² Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin150001, China.

Abstract

Long noncoding RNA (LncRNA) XLOC_006390 has been shown to be dysregulated in cancer tissues and regulates cancer growth and development. Nonetheless, the molecular role of lncRNA-XLOC_006390 in colorectal cancer via modulation of miR-296/ONECUT2 axis is still unclear. Against this backdrop, the current study was designed to explore the role of lncRNA-XLOC_006390 in colorectal cancer proliferation and metastasis. The results revealed significant (P < 0.05) overexpression of lncRNA-XLOC_006390 in colorectal cancer tissues and cell lines, and the transcript levels increased with the advancement of the disease. Moreover, its high expression was shown to be associated with poor patient survival. Silencing of lncRNA-XLOC_006390 in colorectal cancer cells significantly (P < 0.05) suppressed their viability via onset of apoptosis and restricted cancer cell migration and invasion. In vivo tumor growth was significantly (P < 0.05) inhibited under lncRNA-XLOC_006390 repression. LncRNA-XLOC_006390 was shown to sponge the expression of miR-296-3p which in turn acted via post-transcriptional suppression of ONECUT 2 transcription factor to regulate the growth of colorectal cancer. Taken together, the results revealed the oncogenic role of lncRNA-XLOC_006390 in colorectal cancer via modulation of miR-296/ONECUT2 axis. The results also point towards its prognostic and therapeutic potential in the treatment of colorectal cancer.