Two years into the COVID-19 pandemic there is still a need for vaccines to effectively control the spread of novel SARS-CoV-2 variants and associated cases of severe disease. Here we report a messenger RNA vaccine directly encoding for a nanoparticle displaying 60 receptor binding domains (RBDs) of SARS-CoV-2 that acts as a highly effective antigen. A construct encoding the RBD of the Delta variant elicits robust neutralizing antibody response, and also provides protective immunity against the Delta variant in a widely used transgenic mouse model. We ultimately find that the proposed mRNA RBD nanoparticle-based vaccine provides a flexible platform for rapid development and will likely be of great value in combatting current and future SARS-CoV-2 variants of concern.
Keywords: K18-hACE2; SARS-CoV-2; delta variant; mRNA vaccine; nanoparticle; neutralizing antibody; receptor-binding domain.
Copyright © 2022 Brandys, Montagutelli, Merenkova, Barut, Thiel, Schork, Trüeb, Conquet, Deng, Antanasijevic, Lee, Valière, Sindhu, Singh and Herold.