Rhizosphere and Straw Return Interactively Shape Rhizosphere Bacterial Community Composition and Nitrogen Cycling in Paddy Soil

Ya-Hui Zhao; Ning Wang; Meng-Kang Yu; Jian-Guang Yu; Li-Hong Xue

doi:10.3389/fmicb.2022.945927

Rhizosphere and Straw Return Interactively Shape Rhizosphere Bacterial Community Composition and Nitrogen Cycling in Paddy Soil

Front Microbiol. 2022 Jul 7:13:945927. doi: 10.3389/fmicb.2022.945927. eCollection 2022.

Authors

Ya-Hui Zhao¹, Ning Wang^{1

2

3}, Meng-Kang Yu^{1

4}, Jian-Guang Yu^{1

2

3}, Li-Hong Xue^{1

2

3}

Affiliations

¹ Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture, P.R. China, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
² School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.
³ Jiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
⁴ Henan Institute of Science and Technology, Xinxiang, China.

Abstract

Currently, how rice roots interact with straw return in structuring rhizosphere communities and nitrogen (N) cycling functions is relatively unexplored. In this study, paddy soil was amended with wheat straw at 1 and 2% w/w and used for rice growth. The effects of the rhizosphere, straw, and their interaction on soil bacterial community composition and N-cycling gene abundances were assessed at the rice maturity stage. For the soil without straw addition, rice growth, i.e., the rhizosphere effect, significantly altered the bacterial community composition and abundances of N-cycling genes, such as archaeal and bacterial amoA (AOA and AOB), nirK, and nosZ. The comparison of bulk soils between control and straw treatments showed a shift in bacterial community composition and decreased abundance of AOA, AOB, nirS, and nosZ, which were attributed to sole straw effects. The comparison of rhizosphere soils between control and straw treatments showed an increase in the nifH gene and a decrease in the nirK gene, which were attributed to the interaction of straw and the rhizosphere. The number of differentially abundant genera in bulk soils between control and straw treatments was 13-23, similar to the number of 16-22 genera in rhizosphere soil between control and straw treatment. However, the number of genera affected by the rhizosphere effect was much lower in soil amended with straw (3-4) than in soil without straw addition (9). Results suggest possibly more pronounced impacts of straw amendments in shaping soil bacterial community composition.

Keywords: bacterial communities; interactively; nitrogen cycling; rhizosphere; straw return.