A Polypyrrole/Nanoclay Hybrid Film for Ultra-Sensitive Cardiac Troponin T Electrochemical Immunosensor

Biosensors (Basel). 2022 Jul 21;12(7):545. doi: 10.3390/bios12070545.


An electrochemical immunosensor based on a nanohybrid film of carboxylated polypyrrole and amine nanoclay was developed for label-free detection of the human cardiac troponin T (cTnT). The nanohybrid film was formed in situ on the surface of the glassy carbon electrode, followed by the covalent immobilization of anti-troponin T antibodies by glutaraldehyde. Morphological and chemical characterizations of the nanohybrid film were performed by scanning electron microscopy and Fourier-transform infrared spectroscopy. Under the optimized conditions, a calibration curve for cTnT in spiked serum was obtained by square wave voltammetry, and a low limit of detection and quantification was achieved (0.35 and 1.05 pg mL-1, respectively). This was the first time that this type of nanohybrid film was used in the development of an immunosensor for cTnT that proved to be a simple and efficient strategy for the manufacture of a label-free electrochemical device that could be applied in the diagnosis of acute myocardial infarction.

Keywords: cardiac troponin T; immunosensor; nanoclay; polypyrrole.

MeSH terms

  • Biosensing Techniques* / methods
  • Electrochemical Techniques / methods
  • Electrodes
  • Humans
  • Immunoassay / methods
  • Limit of Detection
  • Polymers* / chemistry
  • Pyrroles
  • Troponin T


  • Polymers
  • Pyrroles
  • Troponin T
  • polypyrrole