Molecular interplay of an assembly machinery for nitrous oxide reductase

Nature. 2022 Aug;608(7923):626-631. doi: 10.1038/s41586-022-05015-2. Epub 2022 Jul 27.


Emissions of the critical ozone-depleting and greenhouse gas nitrous oxide (N2O) from soils and industrial processes have increased considerably over the last decades1-3. As the final step of bacterial denitrification, N2O is reduced to chemically inert N2 (refs. 1,4) in a reaction that is catalysed by the copper-dependent nitrous oxide reductase (N2OR) (ref. 5). The assembly of its unique [4Cu:2S] active site cluster CuZ requires both the ATP-binding-cassette (ABC) complex NosDFY and the membrane-anchored copper chaperone NosL (refs. 4,6). Here we report cryo-electron microscopy structures of Pseudomonas stutzeri NosDFY and its complexes with NosL and N2OR, respectively. We find that the periplasmic NosD protein contains a binding site for a Cu+ ion and interacts specifically with NosL in its nucleotide-free state, whereas its binding to N2OR requires a conformational change that is triggered by ATP binding. Mutually exclusive structures of NosDFY in complex with NosL and with N2OR reveal a sequential metal-trafficking and assembly pathway for a highly complex copper site. Within this pathway, NosDFY acts as a mechanical energy transducer rather than as a transporter. It links ATP hydrolysis in the cytoplasm to a conformational transition of the NosD subunit in the periplasm, which is required for NosDFY to switch its interaction partner so that copper ions are handed over from the chaperone NosL to the enzyme N2OR.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Bacterial Proteins* / chemistry
  • Bacterial Proteins* / metabolism
  • Bacterial Proteins* / ultrastructure
  • Binding Sites
  • Copper / chemistry
  • Copper / metabolism
  • Cryoelectron Microscopy*
  • Cytoplasm / enzymology
  • Molecular Chaperones / metabolism
  • Nitrous Oxide* / metabolism
  • Oxidoreductases* / chemistry
  • Oxidoreductases* / metabolism
  • Oxidoreductases* / ultrastructure
  • Periplasm / enzymology
  • Protein Binding
  • Protein Conformation
  • Pseudomonas stutzeri* / cytology
  • Pseudomonas stutzeri* / enzymology


  • Bacterial Proteins
  • Molecular Chaperones
  • Copper
  • Adenosine Triphosphate
  • Oxidoreductases
  • nitrous oxide reductase
  • Nitrous Oxide