Improvement of anemia in five dogs with nonregenerative anemia treated with allogeneic adipose-derived stem cells

Takuya Mizuno; Misuzu Inoue; Takeaki Kubo; Yoshihide Iwaki; Kosuke Kawamoto; Kazuhito Itamoto; Satoshi Kambayashi; Masaya Igase; Kenji Baba; Masaru Okuda

doi:10.1016/j.vas.2022.100264

Improvement of anemia in five dogs with nonregenerative anemia treated with allogeneic adipose-derived stem cells

Vet Anim Sci. 2022 Jul 19:17:100264. doi: 10.1016/j.vas.2022.100264. eCollection 2022 Sep.

Authors

Affiliations

¹ Laboratory of Molecular Diagnostics and Therapeutics, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
² Research & Development Center for Cell Therapy, Foundation for Biomedical Research and Innovation at Kobe, Kobe, Hyogo, Japan.
³ Life Sciences Business Division, FUJIFILM Corporation, Kanagawa, Japan.
⁴ Research and Development Section, Anicom Specialty Medical Institute Inc., Kanagawa, Japan.
⁵ Laboratory of Veterinary Small Animal Clinical Science, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
⁶ Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.

Abstract

Background: Nonregenerative anemia is occasionally seen in dogs and can be caused by many factors, among which nonregenerative immune-mediated anemia (NRIMA) and pure red cell anemia are relatively common causes. These are thought to be caused by immune-mediated destruction of the erythroid lineage and are treated with immunosuppressive drugs, but some of them are refractory or recurrent, so new treatments are needed.

Objectives: To examine the efficacy of allogeneic adipose-derived stem cells (ADSCs) for the treatment of nonregerative anemia in dogs.

Methods: ADSCs were administered to total five nonregenerative anemia cases; two NRIMA cases and two suspected NRIMA cases that were refractory to immunosuppressive agents, and one NRIMA case that has not been treated with immunosuppressive agents.

Results: In all cases, anemia was improved, and blood transfusion was no longer necessary.

Conclusions: This study suggests that allogeneic ADSCs may be one of the rescue therapies for the refractory immune-mediated anemia in dogs.

Keywords: Adipose-derived stem cells; Allogenic; Canine; Mesenchymal stem cells; Nonregenerative anemia.