Design and development of novel single multiplex system incorporating 26 rapidly mutating Y-STRs; 26 RM Yplex

Electrophoresis. 2022 Oct;43(18-19):1911-1919. doi: 10.1002/elps.202200099. Epub 2022 Aug 8.


This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.

Keywords: STRs; discrimination; multiplex; paternal; rapidly mutating.

MeSH terms

  • Alleles
  • Chromosomes, Human, Y* / genetics
  • DNA Fingerprinting
  • Forensic Medicine
  • Haplotypes
  • Humans
  • Male
  • Microsatellite Repeats* / genetics