Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

Shanshan Wang; Baohua Tan; Liyao Xiao; Jiekang Zeng; Xinming Zhao; Linjun Hong; Zicong Li; Gengyuan Cai; Enqin Zheng; Ting Gu; Zhenfang Wu

doi:10.1080/15592294.2022.2105052

Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

Epigenetics. 2022 Dec;17(13):2039-2055. doi: 10.1080/15592294.2022.2105052. Epub 2022 Jul 31.

Authors

Shanshan Wang^{1

2}, Baohua Tan^{1

2}, Liyao Xiao^{1

2}, Jiekang Zeng^{1

2}, Xinming Zhao^{1

2}, Linjun Hong^{1

2}, Zicong Li^{1

2

3}, Gengyuan Cai^{1

2}, Enqin Zheng^{1

2}, Ting Gu^{1

2}, Zhenfang Wu^{1

2

3}

Affiliations

¹ National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
² Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
³ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou, Guangdong, China.

Abstract

Skeletal myogenesis is a highly ordered process finely regulated by various factors. Long non-coding RNAs play an important regulatory role in myogenesis via multiple mechanisms. In this study, we identified the lncRNA Gm10561, which was upregulated during myogenic differentiation and is highly expressed in skeletal muscle. Knockdown of Gm10561 inhibited the proliferation and differentiation of C2C12 myoblasts in vitro and muscle growth in vivo. Overexpression of Gm10561 promoted the proliferation and differentiation of both C2C12 myoblasts and porcine muscle satellite cells. Notably, lncRNA Gm10561 is localized in the cytoplasm and competitively bound to miR-432, which directly targets MEF2C and E2F3. It was confirmed that lncRNA Gm10561 regulates the proliferation and differentiation of myoblasts by acting as a sponge of miR-432 to modulate MEF2C and E2F3 expression. Thus, the lncRNA-Gm10561-miR-432-MEF2C/E2F3 axis plays an important role in myogenesis.

Keywords: Skeletal myogenesis; epigenetics; lncRNA; miRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / genetics
Cell Line
Cell Proliferation / genetics
DNA Methylation
MicroRNAs* / genetics
MicroRNAs* / metabolism
Muscle Development / genetics
Muscle, Skeletal / metabolism
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
Swine

Substances

RNA, Long Noncoding
MicroRNAs

Grants and funding

This work was financially supported by the Guangdong Basic and Applied Basic Research Fund: Regional Joint Fund-Youth Fund Project (2020A1515110319), the China Postdoctoral Science Foundation (Program 2021M691075).