PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes

Fan Hu; Chang Li; Yafen Ye; Xuhong Lu; Miriayi Alimujiang; Ningning Bai; Jingjing Sun; Xiaojing Ma; Xiaohua Li; Ying Yang

doi:10.1080/21623945.2022.2091206

PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes

Adipocyte. 2022 Dec;11(1):379-388. doi: 10.1080/21623945.2022.2091206.

Authors

Affiliations

¹ Shanghai Clinical Center for Diabetes, Shanghai Key Clinical Center for Metabolic Disease, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, China.
² Department of Endocrinology, Seventh People's Hospital Affiliated to Shanghai University of TCM, Shanghai, China.

Abstract

PARP12 is a member of poly-ADP-ribosyl polymerase (PARPs), which has been characterized for its antiviral function. Yet its physiological implication in adipocytes remains unknown. Here, we report a central function of PARP12 in thermogenic adipocytes. We show that PARP12 is highly expressed in brown adipose tissue and is mainly localized to the mitochondria. Knockdown of PARP12 in vitro reduced UCP1 expression. In parallel, the deficiency of PARP12 reduced mitochondrial respiration in adipocytes, while overexpression of PARP12 reversed these effects.

Keywords: Adipocytes; mitochondria; poly(ADP-ribose) polymerases; thermogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / metabolism
Adipocytes, Brown / metabolism
Adipose Tissue, Brown* / metabolism
Mitochondria / metabolism
Thermogenesis* / genetics
Uncoupling Protein 1 / genetics
Uncoupling Protein 1 / metabolism

Substances

Uncoupling Protein 1

Grants and funding

This work was supported by the National Key Research and Development Program of China (2019YFA0904501), the National Science Foundation of China (No.81974122) and the Pudong New Area Health Commission clinical characteristic discipline construction project (PWYts2021-13).