Gas pressure is a promising signal readout mode in point-of-care testing for its merits such as rapidity, simplicity, affordability, and no need for sophisticated instrumentation. Herein, a gas pressure sensor for multiplexed detection of pathogenic bacteria was developed on a hydrogel platform. Spherical and square hydrogel pellets prepared by cross-linking of sodium alginate were functionalized with nisin and ConA for the capture of Staphylococcus aureus and Escherichia coli O157:H7, respectively. By using the shape-encoded functional hydrogel pellets and aptamer-modified platinum-coated gold nanoparticles (Au@PtNPs), a dual-molecule recognition mode was established for rapid and specific detection of the two pathogenic bacteria. Au@PtNPs were applied as signal probes to efficiently catalyze the decomposition of H2O2 for generating abundant O2, which was converted into an amplified gas pressure signal. In two closed containers, the significant gas pressure signals were monitored with a portable pressure meter to quantitate the two pathogenic bacteria. The sensor was successfully applied to detect the pathogenic bacteria in various environmental, biological, and food samples. Thus, the proof-of-principle work paves a new avenue for multiplexed detection of pathogenic bacteria with shape-encoded hydrogel pellets combined with gas pressure signal readout.
Keywords: Escherichia coli O157:H7; Staphylococcus aureus; gas pressure sensor; multiplexed detection; shape-encoded functional hydrogel pellets.