25-OH Vitamin D concentrations measured by LC-MS/MS are equivalent in serum and EDTA plasma

Lisa N van der Vorm; Caroline Le Goff; Stéphanie Peeters; Konstantinos Makris; Etienne Cavalier; Annemieke C Heijboer

doi:10.1016/j.steroids.2022.109096

25-OH Vitamin D concentrations measured by LC-MS/MS are equivalent in serum and EDTA plasma

Steroids. 2022 Nov:187:109096. doi: 10.1016/j.steroids.2022.109096. Epub 2022 Aug 2.

Authors

Lisa N van der Vorm¹, Caroline Le Goff², Stéphanie Peeters², Konstantinos Makris³, Etienne Cavalier², Annemieke C Heijboer⁴

Affiliations

¹ Department of Clinical Chemistry, Central Diagnostic Laboratory, Amsterdam University Medical Center, Amsterdam, the Netherlands.
² Department of Clinical Chemistry, University of Liège (ULiege), CHU de Liège, Sart Tilman, Liège, Belgium.
³ Clinical Biochemistry Department, KAT General Hospital, Athens, Greece; Laboratory for Research of the Musculoskeletal System "Th. Garofalidis", Medical School, University of Athens, Athens, Greece.
⁴ Department of Clinical Chemistry, Endocrine Laboratory, Amsterdam Gastroenterology Endocrinology & Metabolism, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands. Electronic address: a.heijboer@amsterdamumc.nl.

PMID: 35931233
DOI: 10.1016/j.steroids.2022.109096

Abstract

In contrast to a recent study reporting an unexpected significant difference for total 25-hydroxyvitamin D (25(OH)D) between serum and EDTA plasma, we demonstrate that concentrations of total 25(OH)D, 25(OH)D₂, 25(OH)D₃ and 24,25(OH)₂D₃ do not differ between matched serum and EDTA plasma samples, using two well-characterized LC-MS/MS methods.

Keywords: 25(OH)D; LC-MS/MS; Plasma; Serum; Vitamin D.

Publication types

Letter

MeSH terms

Calcifediol
Chromatography, Liquid / methods
Edetic Acid
Tandem Mass Spectrometry* / methods
Vitamin D*

Substances

Vitamin D
Edetic Acid
Calcifediol