Efficacy of disinfection procedures performed prior to regenerative endodontic therapy: An integrative review

Aust Endod J. 2023 Aug;49(2):418-427. doi: 10.1111/aej.12670. Epub 2022 Aug 6.

Abstract

The aim of this integrative review was to identify whether the disinfection procedures performed prior to regenerative endodontic treatment were effective on biofilm removal from the root canals. The research was based on PubMed, Latin American and Caribbean Health Sciences Literature (Lilacs) and Scientific Electronic Library Online (SciELO) databases. Four articles were selected; one of the studies was in vivo and the others ex vivo. Different disinfection procedures were studied, characterised mainly by the use of intracanal medication, highlighting the double antibiotic paste, triple antibiotic paste and calcium hydroxide paste. Disinfection ability was evaluated against Enterococcus faecalis and multispecies biofilms by using the fluorescence technique and colony forming unit counting, for 7 to 21 days. Double antibiotic paste and triple antibiotic paste demonstrated excellent antibiofilm activity, unlike CH paste that showed limited disinfection, even when associated with different antimicrobial agents. Triple antibiotic paste was the most effective medication against biofilm.

Keywords: Intracanal medication; biofilm; disinfection; regenerative endodontics; root canal.

Publication types

  • Review

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Infective Agents*
  • Bacitracin
  • Biofilms
  • Calcium Hydroxide / pharmacology
  • Dental Pulp Cavity
  • Disinfection / methods
  • Enterococcus faecalis
  • Framycetin
  • Polymyxin B
  • Regenerative Endodontics*
  • Root Canal Irrigants / pharmacology

Substances

  • Root Canal Irrigants
  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacitracin
  • Polymyxin B
  • Framycetin
  • Calcium Hydroxide