A multifaceted signal recorder of cellular experiences using Cas12a base-editing

Pengfei Zhang; Michelle M Chan

doi:10.1016/j.tibtech.2022.07.009

A multifaceted signal recorder of cellular experiences using Cas12a base-editing

Trends Biotechnol. 2022 Nov;40(11):1279-1281. doi: 10.1016/j.tibtech.2022.07.009. Epub 2022 Aug 3.

Authors

Pengfei Zhang¹, Michelle M Chan²

Affiliations

¹ Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
² Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address: mmchan@princeton.edu.

PMID: 35933268
DOI: 10.1016/j.tibtech.2022.07.009

Abstract

Technological advances have led to the emergence of lineage tracers, but signal recorders for mammalian systems have remained elusive. Kempton et al. have developed a Cas12a base-editing signal recorder capable of capturing diverse signals and operating in various experimental designs. The recorder enables new opportunities to chronicle cellular history.

Keywords: CRISPR-Cas12a; DNA mutation; base editor; molecular recording; signaling.

MeSH terms

Animals
CRISPR-Cas Systems*
Gene Editing*
Mammals