Multiplex Lateral Flow Assay and the Sample Preparation Method for the Simultaneous Detection of Three Marine Toxins

Environ Sci Technol. 2022 Sep 6;56(17):12210-12217. doi: 10.1021/acs.est.2c02339. Epub 2022 Aug 11.

Abstract

A multiplex lateral flow immunoassay (LFA) has been developed to detect the primary marine biotoxin groups: amnesic shellfish poisoning toxins, paralytic shellfish poisoning toxins, and diarrhetic shellfish poisoning toxins. The performance characteristics of the multiplex LFA were evaluated for its suitability as a screening method for the detection of toxins in shellfish. The marine toxin-specific antibodies were class-specific, and there was no cross-reactivity between the three toxin groups. The test is capable of detecting all three marine toxin groups, with working ranges of 0.2-1.5, 2.5-65.0, and 8.2-140.3 ng/mL for okadaic acid, saxitoxin, and domoic acid, respectively. This allows the multiplex LFA to detect all three toxin groups at the EU regulatory limits, with a single sample extraction method and dilution volume. No matrix effects were observed on the performance of the LFA with mussel samples spiked with toxins. The developed LFA uses a simple and pocket-sized, portable Cube Reader to provide an accurate result. We also evaluated the use of this Cube Reader with commercially available monoplex lateral flow assays for marine toxins.

Keywords: domoic acid; lateral flow; marine toxins; okadaic acid; saxitoxin; shellfish.

MeSH terms

  • Animals
  • Bivalvia*
  • Marine Toxins
  • Okadaic Acid
  • Saxitoxin
  • Shellfish / analysis
  • Shellfish Poisoning*

Substances

  • Marine Toxins
  • Okadaic Acid
  • Saxitoxin