Neuroprotection by melatonin against acrylamide-induced brain damage in pinealectomized rats

J Chem Neuroanat. 2022 Nov:125:102143. doi: 10.1016/j.jchemneu.2022.102143. Epub 2022 Aug 8.


The current study aimed to evaluate the neuroprotective effect of exogenous melatonin against acrylamide (ACR)-induced oxidative stress and inflammatory and apoptotic responses in the brain tissues in pinealectomized rats (PINX). ACR is a toxic chemical carcinogen that occurs owing to the preparation of carbohydrate-rich foods at high temperatures or other thermal processes. The rats who underwent pinealectomy and sham pinealectomy were exposed to ACR (25 mg/kg b.w., orally) alone or with exogenous melatonin (10 mg/kg b.w., i.p.) for 21 consecutive days. Alterations of brain oxidant/antioxidant status, dopamine (DA), Brain-Derived Neurotropic Factor (BDNF) inflammatory mediator and apoptosis during exposure to ACR in pinealectomized rats were more than without pinealectomized rats. Histopathological changes were more in brain tissue of pinealectomized rats after ACR administration. Exogenous melatonin treatment in ACR -exposed rats following pinealectomy increased the activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) and improved brain total antioxidant status (TAS) compared to PINX+ACR. Moreover, melatonin suppressed lipid peroxidation, inflammatory pathways and apoptosis in ACR-intoxicated brain tissues. In addition, after exposure to ACR on pinealectomized rats, melatonin treatment ameliorated BDNF and DA levels in brain tissues. Furthermore, exogenous melatonin intervention in ACR-intoxicated rats significantly rescued the architecture of neuronal tissues. In summary, the present study, for the first time, suggested that exogenous melatonin treatment could reduce oxidative damage by increasing the activities of antioxidant enzymes, inhibiting lipid peroxidation and inflammation, and improving histopathological alterations in the brain tissue of pinealectomized rats after ACR administration.

Keywords: Acrylamide; Inflammation; Lipid peroxidation; Melatonin; Neurotoxicity; Pinealectomy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylamide* / toxicity
  • Animals
  • Antioxidants / metabolism
  • Brain* / drug effects
  • Brain* / metabolism
  • Brain-Derived Neurotrophic Factor / metabolism
  • Melatonin* / therapeutic use
  • Neuroprotection
  • Oxidative Stress
  • Pineal Gland / surgery
  • Rats
  • Rats, Wistar


  • Acrylamide
  • Antioxidants
  • Brain-Derived Neurotrophic Factor
  • Melatonin