A method for freezing synchronous mitotic and G1 cells

Exp Cell Res. 1987 Jun;170(2):363-8. doi: 10.1016/0014-4827(87)90313-2.


A modification of the protocol developed by Kawamoto, J C & Barrett, J N, Brain res (1986), in press for freezing primary neuron cultures in a solution containing low sodium and high lactate and potassium concentrations was used to freeze synchronous mitotic and G1 CHO cells. After thawing, the cells behaved as if they had never been frozen with respect to cell growth, cell division, plating efficiency, and hyperthermic sensitivity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Cycle*
  • Cell Line
  • Cell Survival
  • Cricetinae
  • Freezing
  • Mitosis*
  • Preservation, Biological / methods*