Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China

Lin Li; Chuanxiang Qi; Jinming Li; Wenlong Nan; Ying Wang; Xing Chang; Tianying Chi; Mingxia Gong; Da Ha; Jide De; Lifeng Ma; Xiaodong Wu

doi:10.3389/fvets.2022.936581

Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China

Front Vet Sci. 2022 Jul 26:9:936581. doi: 10.3389/fvets.2022.936581. eCollection 2022.

Authors

Lin Li¹, Chuanxiang Qi^{1

2}, Jinming Li¹, Wenlong Nan¹, Ying Wang¹, Xing Chang¹, Tianying Chi¹, Mingxia Gong¹, Da Ha³, Jide De³, Lifeng Ma⁴, Xiaodong Wu¹

Affiliations

¹ China Animal Health and Epidemiology Center, Qingdao, China.
² MOE Joint International Research Laboratory of Animal Health and Food Safety, MOA Key Laboratory of Animal Bacteriology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
³ Xilingol League Animal Disease Prevention and Control Center, Inner Mongolia, China.
⁴ Inner Mongolia Animal Disease Prevention and Control Center, Inner Mongolia, China.

Abstract

Lumpy skin disease (LSD) is a severe disease of bovine characterized by nodules on the skin, mucous membranes, and profuse nasal discharge which causes severe economic losses. In October 2020, an LSD outbreak case was found in Inner Mongolia Autonomous Region, China. A total of 1,206 cattle were sold from the same imported animal quarantine field to 36 farms after the quarantine period finished, and over 30 farmers reported symptoms such as skin scabs found in newly arrived cattle shortly after that. A large-scale LSD outbreak investigation was launched after laboratory diagnosis confirmed LSD. The clinical samples of 1,206 cattle from 36 farms, including 1,206 whole blood, 1,206 oral and nose swabs, and 355 scabs, were collected for the qRT-PCR test. The result showed that 51 whole blood samples (4.23%), 580 swab samples (48.09%), and 350 skin scabs (98.59%) were lumpy skin disease virus (LSDV) positive, 33 of 36 farms were affected. This study aims to provide a basis for LSD epidemiological traceability, movement control, and measures for prevention and control.

Keywords: 2020; China; Inner Mongolia Autonomous Region; LSDV; outbreak; quantitative real-time PCR (qPCR).