Transcriptional regulation by a RecQ helicase

Methods Enzymol. 2022:673:227-249. doi: 10.1016/bs.mie.2022.03.057. Epub 2022 Apr 18.

Abstract

RecQ helicases participate in a variety of DNA metabolic processes through their multiple biochemical activities. In vitro characterization and cellular studies have suggested that RECQ1 (also known as RECQL or RECQL1) performs its diverse functions through specific interactions with DNA and protein partners. We have taken an unbiased approach to determine the contribution of RECQ1 in genome maintenance and as a putative susceptibility factor in breast cancer. Here, we provide methodology to map the genome-wide binding sites of RECQ1 together with the profiling of RECQ1-dependent transcriptome to investigate its role in gene regulation. The described approach will be helpful to develop a mechanistic framework for elucidating critical functions of RECQ1 and other RecQ homologs in distinct chromatin and biological contexts.

Keywords: ChIP-seq; Genomic stability; Helicase; RNA-seq; RecQ; Transcription.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Breast Neoplasms*
  • DNA / chemistry
  • Female
  • Gene Expression Regulation
  • Genetic Predisposition to Disease
  • Humans
  • RecQ Helicases* / genetics
  • RecQ Helicases* / metabolism

Substances

  • DNA
  • RecQ Helicases