A rapid and simple procedure for dissociation of tumor tissue from the human colon

J Cancer Res Clin Oncol. 1987;113(4):400-1. doi: 10.1007/BF00397727.


A rapid procedure for dissociation of colon tumor tissue which combines enzymatic and mechanical methods is described. Using this method a cell suspension almost free of cell aggregates is obtained which is further characterized by high cell yield and excellent cell viability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Separation / methods*
  • Cell Survival
  • Colon / pathology*
  • Colonic Neoplasms / pathology*
  • Culture Techniques
  • Humans
  • Rectal Neoplasms / pathology*