Association of E-Selectin Gene +A561C Polymorphism with Type 2 Diabetes in Chinese Population

Clin Lab. 2022 Aug 1;68(8). doi: 10.7754/Clin.Lab.2021.211045.

Abstract

Background: Diabetes is associated with endothelial cell dysfunction. E-selectin is an endothelial cell adhesion molecule, which is bound for endothelial cell activation. E-selectin gene+A561C polymorphism is associated with many different disorders: essential hypertension, stroke, angina pectoris, coronary heart disease, etc. But the association with type 2 diabetes remains unclear. Therefore, we aimed to investigate the role of E-selectin gene+A561C polymorphism and soluble E-selectin in type 2 diabetes in a Chinese population.

Methods: This study involved 317 patients with type 2 diabetes and 285 normal healthy controls. Genotyping of E-selectin gene+A561C polymorphism was examined by polymerase chain reaction-restricted fragments length polymorphism (PCR-RFLP). Soluble E-selectin was examined by enzyme linked immunosorbent assay (ELISA). Biochemical markers were measured by Roche 7600 Automated Biochemical Analyzer.

Results: We found that C allele frequency in E-selectin A561 C polymorphism of Chinese T2DM group was higher than control group. The level of soluble E-selectin in T2DM group was higher than control group. TC, TG, LDL-C, ApoB, and sE-selectin (soluble E-selectin) in AC and CC genotypes were higher than AA genotype.

Conclusions: Our findings showed that E-selectin +A561C polymorphism was correlated in the Chinese population with type 2 diabetes. C allele and soluble E-selectin may be predisposing factors of Chinese population with type 2 diabetes.

MeSH terms

  • China
  • Diabetes Mellitus, Type 2* / genetics
  • E-Selectin* / genetics
  • E-Selectin* / metabolism
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Polymorphism, Genetic

Substances

  • E-Selectin
  • SELE protein, human