Simultaneous extraction and quantitation of several bioactive amines in cheese and chocolate

J Chromatogr. 1987 Apr 17;392:317-31. doi: 10.1016/s0021-9673(01)94276-1.


A method is described for simultaneous extraction and quantitation of the amines 2-phenylethylamine, tele-methylhistamine, histamine, tryptamine, m- and p-tyramine, 3-methoxytyramine, 5-hydroxytryptamine, cadaverine, putrescine, spermidine and spermine. This method is based on extractive derivatization of the amines with a perfluoroacylating agent, pentafluorobenzoyl chloride, under basic aqueous conditions. Analysis was done on a gas chromatograph equipped with an electron-capture detector and a capillary column system. The procedure is relatively rapid and provides derivatives with good chromatographic properties. Its application to analysis of the above amines in cheese and chocolate products is described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / analysis*
  • Cacao / analysis*
  • Cheese / analysis*
  • Chromatography, Gas
  • Indicators and Reagents
  • Plants, Edible / analysis*


  • Amines
  • Indicators and Reagents