Capillary gas chromatographic analysis of amino acids in blood and protein hydrolysates as tert.-butyldimethylsilyl derivatives

J Chromatogr. 1987 Apr 24;416(1):15-23. doi: 10.1016/0378-4347(87)80480-2.

Abstract

A method is given for a one-step derivatization and gas chromatography of amino acids in blood and protein hydrolysates. Blood samples are partially purified by solvent extraction. Protein hydrolysates are neutralized with a triethylamine solution. Then tert.-butyldimethylsilyl derivatives of the amino acids are prepared in a one-step procedure and separated on a 30-m fused-silica SE-30 capillary column. Except for tryptophan and cystine, amino acids are eluted within 30 min. Amino acids are derivatized more rapidly than their corresponding trimethylsilyl derivatives and do not degrade on the long fused-silica columns.

MeSH terms

  • Amino Acids / analysis*
  • Amino Acids / blood
  • Animals
  • Caseins / analysis
  • Cattle
  • Chromatography, Gas
  • Collagen / analysis
  • Protein Hydrolysates / analysis*
  • Silicon / analysis

Substances

  • Amino Acids
  • Caseins
  • Protein Hydrolysates
  • trypticase-soy broth
  • Collagen
  • Silicon