Phosphorylation of XPD drives its mitotic role independently of its DNA repair and transcription functions

Sci Adv. 2022 Aug 19;8(33):eabp9457. doi: 10.1126/sciadv.abp9457. Epub 2022 Aug 17.

Abstract

The helicase XPD is known as a key subunit of the DNA repair/transcription factor TFIIH. However, here, we report that XPD, independently to other TFIIH subunits, can localize with the motor kinesin Eg5 to mitotic spindles and the midbodies of human cells. The XPD/Eg5 partnership is promoted upon phosphorylation of Eg5/T926 by the kinase CDK1, and conversely, it is reduced once Eg5/S1033 is phosphorylated by NEK6, a mitotic kinase that also targets XPD at T425. The phosphorylation of XPD does not affect its DNA repair and transcription functions, but it is required for Eg5 localization, checkpoint activation, and chromosome segregation in mitosis. In XPD-mutated cells derived from a patient with xeroderma pigmentosum, the phosphomimetic form XPD/T425D or even the nonphosphorylatable form Eg5/S1033A specifically restores mitotic chromosome segregation errors. These results thus highlight the phospho-dependent mitotic function of XPD and reveal how mitotic defects might contribute to XPD-related disorders.

MeSH terms

  • DNA Helicases / metabolism
  • DNA Repair*
  • Humans
  • NIMA-Related Kinases / genetics
  • Phosphorylation
  • Transcription Factor TFIIH / genetics
  • Transcription Factor TFIIH / metabolism
  • Xeroderma Pigmentosum Group D Protein / genetics
  • Xeroderma Pigmentosum Group D Protein / metabolism*

Substances

  • Transcription Factor TFIIH
  • NEK6 protein, human
  • NIMA-Related Kinases
  • DNA Helicases
  • Xeroderma Pigmentosum Group D Protein
  • ERCC2 protein, human