Objective: To investigate the effects and mechanism of bromodomain and extra-terminal (BET) inhibitor JQ1 on the double-expressor lymphoma (DEL) cell lines.
Methods: Protein expressions of cMyc and BCL-2 in 3 lymphoma cell lines were checked by Western blot so as to identify DEL cell lines. CCK-8 assay was used to detect the effects of JQ1 on anti-proliferation in the DEL cell lines. Western blot and RT-PCR were used to measure the protein and mRNA expressions of cMyc, BCL-2 and BCL-6 in DEL cell lines which treated by JQ1. Flow cytometry was used to detect the effect of JQ1 on cell apoptosis.
Results: Based on the expressions of cMyc and BCL-2, the SU-DHL6 and OCILY3 cell lines were confirmed as DEL cell lines. CCK-8 assay showed that the proliferation of DEL cell lines was inhibited by JQ1, which was similar to non-DEL cell lines and mainly regulated the expression of cMyc and BCL-6 but not BCL-2. JQ1 had no effects on apoptosis in the DEL cell lines.
Conclusion: BET inhibitor JQ1 has anti-tumor effect in the DEL cell lines, thus providing evidence for the therapeutic potential of BET inhibitor JQ1.
题目: BET抑制剂JQ1对双表达淋巴瘤细胞株的作用及其机制研究.
目的: 探讨BET抑制剂JQ1对于双表达B细胞淋巴瘤细胞株的作用机制。.
方法: Western blot检测B细胞淋巴瘤细胞株SU-DHL6、OCILY3、OCILY10 的cMyc和BCL-2蛋白的表达情况，识别双表达淋巴瘤细胞株。CCK-8方法检测JQ1对淋巴瘤细胞株增殖的影响，Western blot及RT-RCR方法检测JQ1对cMyc、BCL-2、BCL-6 mRNA及蛋白水平的影响，流式细胞术检测JQ1对淋巴瘤细胞株凋亡的影响。.
结果: SU-DHL6、OCILY3同时表达cMyc和BCL-2，为双表达淋巴瘤细胞株。CCK-8结果显示，JQ1对双表达淋巴瘤细胞株的增殖有抑制作用，并且JQ1主要调节cMyc、BCL-6的表达，对BCL-2无明显影响，且对B淋巴瘤细胞株凋亡无影响。.
结论: BET抑制剂JQ1可调节cMyc、BCL-6表达，抑制B细胞淋巴瘤增殖。.
Keywords: JQ1; Myc; double-expressor lymphoma.