Novel intrinsic factor Yun maintains female germline stem cell fate through Thickveins

Stem Cell Reports. 2022 Sep 13;17(9):1914-1923. doi: 10.1016/j.stemcr.2022.07.014. Epub 2022 Aug 18.


Germline stem cells (GSCs) are critical for the reproduction of an organism. The self-renewal and differentiation of GSCs must be tightly controlled to avoid uncontrolled stem cell proliferation or premature stem cell differentiation. However, how the self-renewal and differentiation of GSCs are properly controlled is not fully understood. Here, we find that the novel intrinsic factor Yun is required for female GSC maintenance in Drosophila. GSCs undergo precocious differentiation due to de-repression of differentiation factor Bam by defective BMP/Dpp signaling in the absence of yun. Mechanistically, Yun associates with and stabilizes Thickveins (Tkv), the type I receptor of Dpp/BMP signaling. Finally, ectopic expression of a constitutively active Tkv (TkvQD) completely suppresses GSC loss caused by yun depletion. Collectively, these data demonstrate that Yun functions through Tkv to maintain GSC fate. Our results provide new insight into the regulatory mechanisms of how stem cell maintenance is properly controlled.

Keywords: BMP signaling; Drosophila; Germline stem cell; Tkv; Yun; niche; ovary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / physiology
  • Drosophila / metabolism
  • Drosophila Proteins* / genetics
  • Drosophila Proteins* / metabolism
  • Female
  • Germ Cells
  • Intrinsic Factor / metabolism
  • Oogonial Stem Cells* / metabolism
  • Ovary / metabolism
  • Protein Serine-Threonine Kinases
  • Receptors, Cell Surface / metabolism


  • Drosophila Proteins
  • Receptors, Cell Surface
  • dpp protein, Drosophila
  • Intrinsic Factor
  • tkv protein, Drosophila
  • Protein Serine-Threonine Kinases