Comprehensive analysis of lncRNA expression profiles in postmenopausal osteoporosis

Tianning Chen; Kailun Huo; Dece Kong; Shan Su; Tieyi Yang; Weiwei Zhang; Jin Shao

doi:10.1016/j.ygeno.2022.110452

Comprehensive analysis of lncRNA expression profiles in postmenopausal osteoporosis

Genomics. 2022 Sep;114(5):110452. doi: 10.1016/j.ygeno.2022.110452. Epub 2022 Aug 19.

Authors

Tianning Chen¹, Kailun Huo¹, Dece Kong², Shan Su¹, Tieyi Yang², Weiwei Zhang³, Jin Shao⁴

Affiliations

¹ Graduate School of Ningxia Medical University, Yinchuan, Ningxia Hui-Autonomous Region, 750004, China.
² Department of Orthopedics, Pudong New Area Gongli Hospital, School of Clinical Medicine, Shanghai University, Shanghai 200135, China; Research Laboratory of Sports and Health, Institute of Medical Engineering, Shanghai University, Shanghai 200135, China.
³ Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. Electronic address: zhangweiw1980@126.com.
⁴ Department of Orthopedics, Pudong New Area Gongli Hospital, School of Clinical Medicine, Shanghai University, Shanghai 200135, China; Research Laboratory of Sports and Health, Institute of Medical Engineering, Shanghai University, Shanghai 200135, China. Electronic address: shaojin_1977@126.com.

PMID: 35988655
DOI: 10.1016/j.ygeno.2022.110452

Abstract

To explore the key lncRNAs affecting postmenopausal osteoporosis (PMOP) progression, the transcriptome sequencing of peripheral blood mononuclear cells from fifteen early postmenopausal women, according to bone mineral density, were divided into groups of osteoporosis, osteopenia and normality, in each of which the expression profiles of lncRNAs was investigated. From the results we observed nine candidates of lncRNAs, which were to be compared with miRBase, and found that MIR22HG as one candidate of lncRNA was most likely to be directly used as miRNA precursor. Based on the KEGG annotation and lncRNA-miRNA-mRNA-KEGG network, we analyzed the potential role of candidate lncRNAs. The results showed that the expression profiles of lncRNAs could help identify the novel ones involved in the progression of PMOP, and that MIR22HG could serve as a miRNA precursor to regulate FoxO signaling pathway in bone metabolism. Our findings can be of great help in predicting and diagnosing early PMOP.

Keywords: Expression profiles; Postmenopausal osteoporosis; RNA sequencing; lncRNA; mRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Female
Humans
Leukocytes, Mononuclear / metabolism
MicroRNAs* / genetics
MicroRNAs* / metabolism
Osteoporosis, Postmenopausal* / genetics
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism
RNA, Messenger / metabolism

Substances

MicroRNAs
RNA, Long Noncoding
RNA, Messenger