Lytic polysaccharide monooxygenase - A new driving force for lignocellulosic biomass degradation

Bioresour Technol. 2022 Oct:362:127803. doi: 10.1016/j.biortech.2022.127803. Epub 2022 Aug 19.


Lytic polysaccharide monooxygenases (LPMOs) can catalyze polysaccharides by oxidative cleavage of glycosidic bonds and have catalytic activity for cellulose, hemicellulose, chitin, starch and pectin, thus playing an important role in the biomass conversion of lignocellulose. The catalytic substrates of LPMOs are different and the specific catalytic mechanism has not been fully elucidated. Although there have been many studies related to LPMOs, few have actually been put into industrial biomass conversion, which poses a challenge for their expression, regulation and application. In this review, the origin, substrate specificity, structural features, and the relationship between structure and function of LPMOs are described. Additionally, the catalytic mechanism and electron donor of LPMOs and their heterologous expression and regulation are discussed. Finally, the synergistic degradation of biomass by LPMOs with other polysaccharide hydrolases is reviewed, and their current problems and future research directions are pointed out.

Keywords: Biomass conversion; Expression and regulation; Lytic polysaccharide monooxygenase; Reducing agent; Substrate specificity.

Publication types

  • Review

MeSH terms

  • Biomass
  • Lignin*
  • Mixed Function Oxygenases* / metabolism
  • Polysaccharides / chemistry


  • Polysaccharides
  • lignocellulose
  • Lignin
  • Mixed Function Oxygenases