Highly Efficient Use of Infrared Spectroscopy (ATR-FTIR) to Identify Aphid Species

Biology (Basel). 2022 Aug 18;11(8):1232. doi: 10.3390/biology11081232.


Aphids are commonly considered to be serious pests for trees, herbaceous and cultivated plants. Recognition and identification of individual species is very difficult and is based mainly on morphological features. The aims of the study were to suggest the possibility of identifying aphids through the use of Fourier-transform infrared (FTIR) spectroscopy, and to determine which absorption peaks are the most useful to separate aphid species. Using FTIR spectroscopy, based on the chemical composition of the body, we were able to distinguish 12 species of aphid. We have shown that using nine distinct peaks corresponding to the molecular vibrations from carbohydrates, lipids, amides I and II, it is possible to accurately identify aphid species with an efficiency of 98%.

Keywords: chemotaxonomy; entomology; mid-infrared spectroscopy; rapid species identification.