Ultrasensitive Acetylcholinesterase detection based on a surface-enhanced Raman scattering lever strategy for identifying nerve fibers

Tianao Li; Tao Sui; Binyu Wang; Kun Xu; Sheng Zhang; Xiaojian Cao; Yi Wang; Weiping Qian; Jian Dong

doi:10.1016/j.talanta.2022.123867

Ultrasensitive Acetylcholinesterase detection based on a surface-enhanced Raman scattering lever strategy for identifying nerve fibers

Talanta. 2023 Jan 15:252:123867. doi: 10.1016/j.talanta.2022.123867. Epub 2022 Aug 25.

Authors

Tianao Li¹, Tao Sui², Binyu Wang², Kun Xu¹, Sheng Zhang², Xiaojian Cao³, Yi Wang⁴, Weiping Qian⁵, Jian Dong⁶

Affiliations

¹ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
² Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
³ Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China. Electronic address: xiaojiancao001@163.com.
⁴ Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Whenzhou, 325001, China. Electronic address: wangyi@wibe.ac.cn.
⁵ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. Electronic address: wqian@seu.edu.cn.
⁶ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China; Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Whenzhou, 325001, China; Laboratory of Environment and Biosafety, Research Institute of Southeast University in Suzhou, Suzhou, 215123, China. Electronic address: dongjian@seu.edu.cn.

PMID: 36041317
DOI: 10.1016/j.talanta.2022.123867

Abstract

Accurate discriminating nerve fibers is a prerequisite for right suturing nerves in nerve transfer operation. Various methods have been developed for identification of motor and sensory fibers, but no simple method meets the requirements in clinic. In this study, a surface-enhanced Raman scattering (SERS) lever strategy is designed and developed to detect Acetylcholinesterase (AchE) ultrasensitively, in which using produced thiocholine with weak intrinsic Raman activity (four ounces) to adjust absorbance of Rhodamine B with strong intrinsic Raman activity (thousand catties) on SERS-active substrates is to increase the sensitivity. Employing a miniaturized SERS substrate, SERS-active microneedles, is to decrease the volume of enzymolysis systems. Adopting an internal reference is to increase the repeatability of collected signal. The ultrasensitive AchE detection method discriminate samples with four times of difference in enzyme activity between 1-1 × 10^-4 U/mL in about 10 min of enzymolysis time. AchE amounts in 2-mm-long segments of ventral and dorsal roots were about 0.00025-0.001 U and 0.01-0.02 U, respectively. The developed method would be a reliable method met the requirements of identifying motor and sensory fibers in clinic.

Keywords: AchE; Nerve fibers; SERS lever Strategy; SERS-Active microneedle.

MeSH terms

Acetylcholinesterase*
Nerve Fibers
Spectrum Analysis, Raman* / methods
Thiocholine

Substances

Acetylcholinesterase
Thiocholine