Tissue-Characteristic Expression of Mouse Proteome

Mol Cell Proteomics. 2022 Oct;21(10):100408. doi: 10.1016/j.mcpro.2022.100408. Epub 2022 Sep 2.


The mouse is a valuable model organism for biomedical research. Here, we established a comprehensive spectral library and the data-independent acquisition-based quantitative proteome maps for 41 mouse organs, including some rarely reported organs such as the cornea, retina, and nine paired organs. The mouse spectral library contained 178,304 peptides from 12,320 proteins, including 1678 proteins not reported in previous mouse spectral libraries. Our data suggested that organs from the nervous system and immune system expressed the most distinct proteome compared with other organs. We also found characteristic protein expression of immune-privileged organs, which may help understanding possible immune rejection after organ transplantation. Each tissue type expressed characteristic high-abundance proteins related to its physiological functions. We also uncovered some tissue-specific proteins which have not been reported previously. The testis expressed highest number of tissue-specific proteins. By comparison of nine paired organs including kidneys, testes, and adrenal glands, we found left organs exhibited higher levels of antioxidant enzymes. We also observed expression asymmetry for proteins related to the apoptotic process, tumor suppression, and organ functions between the left and right sides. This study provides a comprehensive spectral library and a quantitative proteome resource for mouse studies.

Keywords: antioxidant enzymes; data independent acquisition; immune privilege; laterality; mouse; multi-organ; proteome; spectral library; testis; tissue-specific proteins.

MeSH terms

  • Animals
  • Antioxidants*
  • Male
  • Mice
  • Peptides
  • Proteome*
  • Proteomics


  • Proteome
  • Antioxidants
  • Peptides