Development of a high specificity typing method for the detection of herpes simplex virus

Zhu Chen; Kaixuan Zhao; Boyu Tan; Zengrui Tong; Ziyu He; Xiaofang Luo; Lei Cai; Hanming Wang; Polly H M Leung; Franklin Wang-Ngai Chow; Hui Chen; Yan Deng

doi:10.3389/fbioe.2022.955713

Development of a high specificity typing method for the detection of herpes simplex virus

Front Bioeng Biotechnol. 2022 Aug 17:10:955713. doi: 10.3389/fbioe.2022.955713. eCollection 2022.

Authors

Zhu Chen¹, Kaixuan Zhao¹, Boyu Tan¹, Zengrui Tong¹, Ziyu He², Xiaofang Luo³, Lei Cai⁴, Hanming Wang⁵, Polly H M Leung⁶, Franklin Wang-Ngai Chow⁶, Hui Chen¹, Yan Deng¹

Affiliations

¹ Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, China.
² Department of Scientific Research, Zhuzhou Hospital Affiliated to Xiangya School of Medical, Central South University, Zhuzhou, China.
³ College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou, China.
⁴ Guangzhou Wondfo iCubate Biotech Co. Ltd., Guangzhou, China.
⁵ Wondfo Biotech Co. Ltd., Guangzhou, China.
⁶ Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China.

Abstract

Herpes disease is caused by Herpes simplex virus (HSV). It has become one of the global health problems. This paper reports a method for HSV type testing. First specific primers sequence for HSV-1 and HSV-2 were selected, designed, and synthesized. Then, these amplification products were proved by sequencing and analysis. Lastly, we optimized the reaction system and PCR reaction program by orthogonal design and sensitivity testing. Results showed that the lowest concentration in HSV-type testing is about 6.67 × 10⁶ copies/ml. Moreover, the specificity of detection was very high. So, this method has very great potentials for HSV type testing in clinical practice.

Keywords: HSV typing; HSV-1; HSV-2; orthogonal design; primer design.