Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice

STAR Protoc. 2022 Aug 29;3(3):101652. doi: 10.1016/j.xpro.2022.101652. eCollection 2022 Sep 16.


Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior protection against SARS-CoV-2. This protocol encompasses intranasal vaccination in mice, step-by-step bronchoalveolar lavage for both cellular and acellular airway components, lung mononuclear cell isolation, and detailed flow cytometric characterization of lung tissue-resident memory T cell responses, and airway macrophage-trained innate immunity. For complete details on the use and execution of this protocol, please refer to Afkhami et al. (2022).

Keywords: Cell isolation; Flow Cytometry/Mass Cytometry; Immunology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COVID-19* / prevention & control
  • Immunity, Innate
  • Lung
  • Memory T Cells*
  • Mice
  • SARS-CoV-2
  • Vaccination / methods

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