Early plasma membrane depolarization by alpha interferon: biologic correlation with antiproliferative signal

Biochem Biophys Res Commun. 1987 Jul 15;146(1):300-6. doi: 10.1016/0006-291x(87)90725-x.

Abstract

Daudi lymphoma cells, of a line sensitive to growth inhibition by alpha interferon, showed dose-dependent plasma membrane depolarization within 10 min after exposure to natural or recombinant alpha interferons (10 to 1000 IU/ml). This biophysical change was detected flow cytometrically by measuring the intensity of fluorescent emission from cells stained with dye indicators of membrane potential. Subclones of Daudi lymphoma cells, resistant to growth inhibition by alpha interferon, showed no membrane depolarization. Parallel results were obtained in initial tests of an isologous pair of T cell and B cell lines which differ in sensitivity to growth inhibition. Thus, decreased membrane potential may herald an interferon signal for antiproliferative action.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Burkitt Lymphoma / ultrastructure
  • Cell Division / drug effects
  • Cell Line
  • Cell Membrane / drug effects*
  • Flow Cytometry
  • Humans
  • Interferon Type I / pharmacology*
  • Membrane Potentials / drug effects
  • Recombinant Proteins / pharmacology

Substances

  • Interferon Type I
  • Recombinant Proteins