An optimized mouse model of Staphylococcus aureus infected diabetic ulcers

BMC Res Notes. 2022 Sep 7;15(1):293. doi: 10.1186/s13104-022-06170-5.


Objective: Diabetic foot infection (DFI) represents a major healthcare burden, for which treatment is challenging owing to the pathophysiological alterations intrinsic to diabetes and the alarming increase of antimicrobial resistance. Novel therapies targeting DFI are therefore a pressing research need for which proper models of disease are required.

Results: Here, we present an optimized diabetic mouse model of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds, that resemble key features of DFI, such as pathogen invasion through wound bed and surrounding tissue, necrosis, persistent inflammation and impaired wound healing. Thus, in a time-efficient manner and using simple techniques, this model represents a suitable approach for studying emerging therapies targeting DFI caused by MRSA.

Keywords: Chronic wounds; Diabetic mouse model; Impaired wound healing; Inflammation; MRSA infection.

MeSH terms

  • Animals
  • Diabetes Mellitus*
  • Diabetic Foot* / therapy
  • Disease Models, Animal
  • Methicillin-Resistant Staphylococcus aureus*
  • Mice
  • Skin Diseases*
  • Staphylococcal Infections* / complications
  • Staphylococcal Infections* / therapy
  • Staphylococcus aureus
  • Ulcer