Unusual patterns of somatic embryogenesis in the domesticated carrot: developmental effects of exogenous auxins and auxin transport inhibitors

Cell Differ. 1987 Jun;21(1):53-62. doi: 10.1016/0045-6039(87)90448-9.

Abstract

The effects of various exogenous auxins and polar auxin transport inhibitors on somatic embryogenesis in carrot cultures were investigated. Indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid do not disrupt the sequence or the polarity of individual stages in embryo development, but tend to cause developing embryos to revert to undifferentiated callus, with increasing frequency in later embryo stages. The transport inhibitors, N-(1-naphthyl)phthalamic acid and 2,3,5-triiodobenzoic acid, block morphological transitions to the subsequent stage; for example, they cause the formation of enlarged globular and oblong embryos. Heart embryos in these treatments usually develop additional lateral growth axes. These results shed light on the role of auxin and its polar transport in somatic embryogenesis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 2,4-Dichlorophenoxyacetic Acid / pharmacology
  • Biological Transport / drug effects
  • Culture Techniques
  • Indoleacetic Acids / antagonists & inhibitors
  • Indoleacetic Acids / pharmacology*
  • Phthalimides / pharmacology
  • Plant Growth Regulators / pharmacology*
  • Plants / embryology*
  • Triiodobenzoic Acids / pharmacology
  • Vegetables

Substances

  • Indoleacetic Acids
  • Phthalimides
  • Plant Growth Regulators
  • Triiodobenzoic Acids
  • 2,4-Dichlorophenoxyacetic Acid
  • alpha-naphthylphthalamic acid
  • indoleacetic acid
  • 2,3,5-triiodobenzoic acid